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To determine whether persistent infection was present, we swabbed fish K1 to K6 every other day for 1 month to see if we could detect the presence of KHV or KHV DNA on gill surfaces and within the anal vent.
KHV DNA and infectious virus were detected in both gill and fecal swabs by day 8 following temperature stress.
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The human body can store only a certain amount of vitamin C, and so the body stores are depleted if fresh supplies are not consumed.
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Anal vent and gill swabs were collected from all six koi every other day starting at day 2 post-temperature increase until day 16 post-temperature stress.
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The effect of vitamin C on the common cold has been extensively researched.
To investigate whether KHV becomes latent in certain tissues, all koi in this study were euthanized via MS ppm overdose.